You are provided with a sample of crude amylase enzyme. Describe different methods used for concentration and purification of this enzyme.?
Answers
hcbiochem
You could concentrated it by precipitating the protein with ammonium sulfate or alcohol and dissolving the precipitated protein in a smaller volume. You could purify the enzyme using any of the various types of chromatography: gel filtration, ion exchange or affinity chromatography.
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